Enzyme Activity Lab

What We Used

So in this lab we had to use a lot of different materials. They are all listed below.
- Computer
- Logger Pro
- Vemier Gas Pressure Sensor
- 1-Hole Rubber Stopper Assembly
- 10 mL Beaker of Water
- 250 mL Beaker of Water
- 3% H2O2
-600 mL Beaker
- Enzyme Suspension
- Four 18 x 150 mm Test Tubes
- Ice
- pH Buffers
- Test Tube Rack
-Thermometer
- Three Dropper pipettes

Introduction

So in this lab we are really focusing on enzymes. An enzyme is a type of protein that speeds up a certain chemical reaction and is produced by a living organism. We are going to try and demonstrate how enzymes works in different conditions and which ones they work best in. By doing so we are using yeast, hydrogen peroxide. water and different substances. In this lab the yeast is being use to demonstrate the enzyme and the hydrogen peroxide will be used to demonstrate substrates. There were kind of like three parts that we had to do differently over the experiment.

 

Procedure 

First Part -So first off we collected four test tubes and put 3 mL of water and 3 mL of hydrogen peroxide in each one. Then I put 15 drops of yeast which was the enzyme, then  on one test tube I attached the gas pressure sensor to the tube and kind of swirled the tube to mix the contents. We had to wait a little before we were able to get our results. Once we got our resulted we recorded it and had to do this three more times for our remainder tubes. Instead of doing 15 drops of yeast we ended up increasing the amount of drop for each tube to 22, 30, then 40 for our last one.

 

15 Drops- 21.49 kPa/min

22 Drops- 51.69 kPa/min

30 Drops- 10.12 kPa/min

40 Drops- 56.52 kPa/min



Second Part- We collected four test tubes, added 3 mL of water and 3 mL of hydrogen peroxide in each one of the tubes. In this part we decided to change up the temperatures of each tube by putting them in either ice or boiling water. There different temperatures were measured at 0 degree Celsius, 20 degree Celsius, 55 degree Celsius, and 99 degree Celsius. We let each of the test tubes sit for about 10 minutes then we added the same amount of drops of yeast (enzyme)  to each tube and again connected the gas pressure sensor. We had to wait a little before we were able to record our results.

0 Celsius- 26.16 kPa/min

20 Celsius- 28.8 kPa/min

55 Celsius- 36.59 kPa/min

90 Celsius- 58.369 kPa/min

Part Three- For the last part it was a little easier, we collected only three test tubes and added 3 mL of hydrogen peroxide into each one of them. Then we added 3 mL of  pH 4 in the first test tube, in the second one we put 3 mL pH 7 and in the last test tube we put 3 mL of pH 10. For the last time we attached the gas pressure sensor to the test tube and gently shook it to mix the contents. We waited a little then we able to get our results to record.

 

 

pH 4- 0.315 kPa/min

pH 7- 0.259 kPa/min

pH 10- 0.387 kPa/min

 

Conclusion

Now to wrap all of this up, we ended up studying the different rates of enzyme in their different temperatures. As the environment for the enzymes changes so did the rate for the reactions which was to be expected. In the first part the highest slope was 40 drops with a slope of 56.52 kPa/min. In the second part the highest slope was the 90 degree Celsius with 58.369 kPa/min. So in the last part the highest slope ended up being the pH 10 which was 0.387 kPa/min.