1. Place 1 gram of raw wheat germ in a 50 ml test tube
2. Add 20 ml of hot (50-60 Celsius Degrees) tap water and mix constantly for three minutes.
3. Add 1 ml or a scant 1/4 teaspoon of detergent and mix gently every minute for 5 minutes. Try not to create foam.
4. Us an eye dropper, pipette, or piece of paper towel to remove any foam from the top of the solution.
5 Tilt the test tube at an angle. Slowly pout 14 ml of alcohol down the side so that it forms a layer on top of the water wheat germ/ detergent solution. Do not ix the two layers together . DNA precipitates at the water- alcohol interface (the boundary between the water and the alcohol). Therefore, it is crucial to pour the alcohol very slowly so that it forms a layer on top of the water solution. If the alcohol mixes with the water, it will become too dilute and the DNA will not precipitate.
6. Let the test tube sit for a few minutes. White, stringy, filmy DNA will begin to appear where the water and alcohol meet. You will usually see DNA precipitating from the solution at the water-alcohol interface as soon as you pour in the alcohol. If you let the preparation sit for 15 minutes or so, the DNA will float to the top of the alcohol.
7. Use a glass or a paper clip hook or a wooden stick to collect the DNA.
Observation
This is when I added alcohol and it didn't mix.
No comments:
Post a Comment