Sunday, April 20, 2014

Gel electrophoresis


Procedure 
1. First we had to make the gel substance. By doing so we had to measure the amount of agarose (gel stuff0 about three grams then add it to water. We mixed the water up and put it in the microwave to heat it up. It was just like jell-o, in order for it to become a gel substance it had to be heated up. So after it was heated we added it to the trays and let it sit there over night. Once we came back to school the next day we were able to actually start the project. There were multiple stations and we split up in to different groups. There were many tools that were sterile. So we grabbed a pipette and measured the right about of substance and put it in the little squares in the gel. While doing this we had to make sure we didn't go through the gel otherwise it would make a mess. Once you knew you were in the gel you would press down and the substance would release. This was kind of a ruff process. Afterwards we put the lid on and hooked it up to the gel electrophoresis (gel rig). We left it going for about 45 minutes.
So in the end they liquid dyes had spread out and some of them had reacted differently because the mixtures had been different.
There are pictures of the different steps I had went through and the reactions the had happened. While putting the dye I had difficulties of not going through the gel and on some of them I had. In the pictures you will see how it spread out.







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